Regulatory

Part:BBa_K1820008

Designed by: Alex Torgesen   Group: iGEM15_Utah_State   (2015-09-16)

CP8 promoter

Synthetic promoter created for use in Lactococcus lactis but also shows activity in E. coli, part BBa_K1033220 from the 2013 Uppsala team. Of the three promoters that we chose to use; cp8, cp11, and cp44; this one had the highest activity. It does not contain a ribosomal binding site. The paper listed below has more information.

Jensen, Peter Ruhdal and Karin Hammer. "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." Applied and Environmental Microbiology (1998): 82-87. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/]

To visit the original submission of this part by the 2013 Uppsala team, click here: https://parts.igem.org/Part:BBa_K1033220

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None